Table 2 System suitability, LOD, LOQ and the concentration for 9 analytes
Analytes | Theoretical plate | Resolution | Tailing factor | LOD (µg/mL) | LOQ (µg/mL) | sample 1 (mg/g) | sample 2 (mg/g) | sample 3 (mg/g) | Mean (n = 3) | RSD (%) |
|---|---|---|---|---|---|---|---|---|---|---|
Dracorhodin perchlorate | 9233 | 1.74 | 1.416 | 0.37 | 1.29 | 3.42 | 3.54 | 3.41 | 3.46 | 2.18 |
Aloe-emodin | 44,145 | 1.6 | 1.033 | 0.25 | 0.93 | 0.81 | 0.85 | 0.82 | 0.82 | 2.85 |
Rhein | 50,103 | 3.35 | 1.082 | 0.27 | 1.13 | 1.04 | 1.07 | 1.03 | 1.05 | 1.74 |
Imperatorin | 103,352 | 2 | 1.028 | 0.44 | 1.64 | 0.89 | 0.9 | 0.86 | 0.88 | 2.17 |
Emodin | 98,885 | 1.48 | 1.045 | 0.69 | 1.39 | 1.95 | 2.03 | 1.96 | 1.98 | 2.12 |
Isoimperatorin | 187,272 | 2.19 | 1.033 | 0.13 | 0.44 | 0.58 | 0.58 | 0.58 | 0.58 | 0.41 |
Chrysophanol | 271,269 | 2.2 | 1.041 | 0.22 | 0.73 | 4.45 | 4.65 | 4.47 | 4.52 | 2.48 |
Physcion | 270,813 | 1.49 | 1.069 | 0.36 | 1.32 | 1.24 | 1.29 | 1.25 | 1.26 | 2.27 |
Tanshinone IIA | 225,496 | 4.05 | 1.046 | 0.14 | 0.45 | 0.57 | 0.6 | 0.57 | 0.58 | 2.87 |