Cytotoxicity of the methanol extracts and compounds of Brucea antidysenterica (Simaroubaceae) towards multifactorial drug-resistant human cancer cell lines

Table 2 IC₅₀ values of crude extracts from leaves (BAL), stem (BAS) of Brucea antidysenterica, and doxorubicin towards the tested cell lines

Cell lines	Samples, IC₅₀ ( µg/mL) and D.R.* or S.I.** (in bracket)
Cell lines	Crude extracts		Reference drug
	BAL	BAS	Doxorubicin
CCRF-CEM	17.42 ± 0.84	43.42 ± 2.86	0.02 ± 0.00
CEM/ADR5000 Degree of resistance*	18.84 ± 1.28 (1.08)	12.43 ± 0.68 (0.29)	122.96 ± 10.94 (6,683.00)
MDA-MB-231-pcDNA	36.34 ± 1.99	34.76 ± 1.78	0.13 ± 0.01
MDA-MB-231-BCRP Degree of resistance	18.67 ± 1.53 (0.51)	68.12 ± 3.47 (1.96)	0.79 ± 0.08 (6.14)
HCT116 p53^+/+	37.18 ± 2.51	68.91 ± 4.69	0.48 ± 0.06
HCT116 p53^−/− Degree of resistance	38.70 ± 3.02 (1.04)	> 80 (> 1.16)	1.78 ± 0.08 (3.73)
U87MG	36.83 ± 2.55	34.21 ± 1.30	0.26 ± 0.03
U87MG.ΔEGFR Degree of resistance	25.57 ± 1.84 (0.69)	55.18 ± 3.82 (1.61)	0.98 ± 0.07 (3.79)
HepG2	36.98 ± 2.41	28.61 ± 0.97	4.56 ± 0.48
AML12 Selectivity index**	78.39 ± 4.62 (2.12)	> 80 (> 2.80)	> 50 (> 11.59)

(*): Degree of resistance (D.R.): ratio of IC₅₀ vs IC₅₀ in the sensitive cell line; CEM/ADR5000 vs CCRF-CEM, MDA-MB-231-BCRP vs MDA-MB-231-pcDNA, HCT116 p53^−/− vs HCT116 p53^+/+ and U87.MGΔEGFR vs U87.MG; (**): Selectivity index (S.I): ratio of IC₅₀ in AML12 vs IC₅₀ in HepG2 cells [13, 48]; Methanol extract from leaves (BAL), and stem (BAS) of Brucea antidysenterica. In bold: significant cytotoxic effect [9, 72]

ISSN: 2662-7671