Table 1 Primers used for real-time RT-PCR
Genes | Primer sequence (5´-3´ orientation) | Annealing temp (°C) | Product size (bp) | Gene bank accession no |
|---|---|---|---|---|
DNMT3a | F: TGATGGGATTGCTACAGGGCT | 62 | ||
R: ATGCTTCTGTGTGACGCTGC | 163 | NM_007872.4 | ||
DNMT3b | F: CCAGCCTCACGACAGGAAACA | 62 | 78 | NM_001271747.1 |
R: CTCCTCATACCCGCTGGCAC | ||||
DNMT1 | F:GACAGTGACACCCTTTCAGTTG | 64 | 94 | NM_001199431.2 |
R: CCTTCGTGAAGTGAGCCGTG | ||||
HDAC1 | F: ACGGCATTGACGACGAATCCT | 64 | 158 | NM_008228.3 |
R: GCGTGTCCTTTGATGGTCAGA | ||||
HDAC2 | F: ACGGGTGGTTCAGTTGCTGG | 64 | 77 | NM_008229.2 |
R: AGTCCTCCAGCCCAATTGACAG | ||||
Estrogen receptor 1 (alpha):ESR1 | F: ATTCTTCTCAAGCAGGTGGCCC | |||
R: GCTCCAGCTCGTTCCCTTGG | 61 | 159 | NM_007956.5 | |
Estrogen receptor 2 (beta): ESR2 | F: GAGTGCTGTCCCAAGGGATGA | |||
R: CGCCAGGAGCATGTCAAAGAT | 57 | 70 | NM_207707.1 | |
-βactin | F: GTCCACACCCGCCACCAGTT | 65 | NM_007393.5 | |
R: GAGCCGTTGTCGACGACCAG | 60 |