Fig. 1

Effects of L-glutamate on C6 cell viability. a MTT assay showed that cell survival is dependent on glutamate concentration. AO/PI results were compatible with the MTT assay. Increased L-glutamate concentration led to significantly decreased cell viability. The data are expressed as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001. b Observation of cells under the inverted microscope with a 20X c) AOPI fluorescence images of C6 cells in the presence of different glutamate concentrations