Fig. 2

Effects of L-AP pre-treatment on LPS-induced neuroinflammation in BV-2 cells. A Bright-field micrographs showing BV-2 morphology (×250); B, C Effect of L-AP on cell viability in the absence or presence of LPS stimulation in BV-2 cells by CCK8 assay; D Bright-field micrographs showing the effect of LPS on BV-2 morphology, the red arrow indicates activated BV-2 cell (×250); E The protein concentrations of IL-6 and TNF-α were assayed by ELISA; F mRNA levels of inos, il-10 and arg-1 in BV-2 cells. Values are presented as mean ± S.E.M. ^*p < 0.05, versus ctrl group (n = 5). ^#p < 0.05, versus LPS alone treatment group (n = 5). ^&p < 0.05, versus VitC group (n = 5)