Fig. 6

Photomicrographs depicting ICAM-1 stained liver sections at 400X magnifications (I A) and, ICAM-1 stained spleen sections at 200X magnifications (II A) of mice treated with Chalcone derivatives 1 (10 mg/kg), 2 (20 mg/kg) and 3 (10 mg/kg) using Chloroquine diphosphate (10 mg/kg) as standard chloroquine sensitive antimalarial drug in P. berghei NK-65 infected mouse model for five days. Biotin-conjugated secondary antibody and streptavidin-conjugated horseradish peroxidase from DAB Substrate kit ( Scy Tek) were applied to sections to amplify the antigen signal for subsequent 3,3-diaminobenzidine staining, which produces a permanent brown color. a) Non-infected Control, b) Infected Control c) Chloroquine-Treated (10 mg/kg) d) Chalcone Derivative 1 Treated (10 mg/kg), e) Derivative 2 Treated (20 mg/kg) and f) Derivative 3 Treated (20 mg/kg). Bar scale represent 100 μm. (I B & II B) ICAM-1 expression in liver section of mice and, ICAM-1 expression in spleen section of mice. Pearson Chi-square, contingency co-efficient, correlations test was applied for statistical significance (*p < 0.05)