Table 1 Primers sequences designed for Real-time PCR
From: Classical music restored fertility status in rat model of premature ovarian failure
Gene/ NCBI accession number | Sequence (5’→3’) | Annealing Temperature (˚C) | |
|---|---|---|---|
Ntrk2 NM_001163168.2 | F | ACCTGCGGCACATCAATTTC | 60 |
R | ACAAATCCTGAGTGTCGGGG | 60 | |
Crh NM_031019.2 | F | CTGATCCGCATGGGTGAAGA | 60 |
R | GGAAAAAGTTAGCCGCAGCC | 60 | |
Pomc NM_139326.3 | F | ATAGACGTGTGGAGCTGGTG | 60 |
R | CGGAAGTGACCCATGACGTA | 60 | |