Table I List of primer pairs used in real-time PCR
Target genes | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
GAPDH | CTCCTCCACCTTTGACGCTG | TCCTCTTGTGCTCTTGCTGG |
Cyclin A | GCAAACAGTAAACAGCCTGCGTTC | TGGGTCCAGGTAAACTAATGGCTG |
Cyclin B | GGAGAGGTTGATGTCGAGCAAC | GAGAAGGAGGAAAGTGCACCATG |
CDK1 | AGTCTTCAGGATGTGCTTATGCAG | AGAATCCATGTACTGACCAGGAGG |
Bcl2 | GTGGCCTTCTTTGAGTTCGGTG | GTGCCGGTTCAGGTACTCAGTC |
Survivin | GGACCACCGCATCTCTACATTC | ATGGGGTCGTCATCTGGCTC |
Snail | TTACCTTCCAGCAGCCCTACGA | GAGCCTTTCCCACTGTCCTCAT |
MMP2 | ATAACCTGGATGCCGTCGT | AGGCACCCTTGAAGAAGTAGC |
FAK | ATCCCACACATCTTGCTGACTT | GCATTCCTTTTCTGTCCTTGTC |
E-cadherin | GAAGGAGGCGGAGAAGAGGAC | CGTCGTTACGAGTCACTTCAGG |
N-cadherin | AGGTGGAGGAGAAGAAGACCAGG | GATGGCATCAGGCTCCACAGT |