Fig. 2

Induction of cell cycle arrest in cervical cancer cells after MNBE treatment. (A) HeLa, SiHa, and ME-180 cells were harvested after treatment with MNBE at the indicated concentration for 24 h. The distribution of the cell cycle was analyzed by flow cytometry using PI staining. The percentages of cells in the G1/G0, S, and G2/M phases were calculated using FlowJo software. (B) Quantitative real-time PCR analysis of cyclin A, cyclin B, and CDK1 after cells were treated with MNBE for 24 h. Data expressed as the mean ± SEM of the three independent experiments. Vehicle-treated cells were used as control. The significant differences from control are indicated by *P < 0.05, **P < 0.01, ***P < 0.001 and **** P < 0.0001 vs vehicle control