Fig. 1

The antiproliferative effect of MNBE on cervical cancer cell lines. (A) HeLa, SiHa, ME-180, and non-cancerous L2 cells (10⁴ cells/well) were seeded into a 96-well plate, incubated overnight, and then treated with the 0–300 µg/ml of MNBE for 24 h. Viability was measured by MTT assay, and the viability of untreated cells was set at 100%. Results graphed using the log (inhibitor) vs. response—Variable slope (four parameters) curve using GraphPad Prism. (B) HeLa, SiHa, and ME-180 cells were seeded in 60 mm dishes in a range of 200–1000 cells and allowed to grow for 24 h. Cells were then treated with corresponding IC₂₅ and IC₅₀ doses of MNBE for 24 h. After treatment, media was replaced with a fresh medium, and cells were incubated for 10–14 days. Cells were fixed in 4% paraformaldehyde for 20 min and stained with 0.4% crystal violet for 15 min. The stained cells were viewed and counted under a microscope. All experiments were performed in triplicate, and data are expressed as the mean ± SEM of the three independent experiments. Vehicle-treated cells were used as control. **** P < 0.0001 vs vehicle control