Fig. 6

MMP-7 is expressed by the CD45⁺ infiltrating immune cells, Ly6G⁺ neutrophils, and F4/80⁺ macrophages in the DSS-induced colitis mouse model. The immunofluorescence double-staining images of colonic tissues in DSS-induced colitis mice. Expression of (A) MMP-7 and CD45, (C) MMP-7 and Ly6G, and (E) MMP-7 and F4/80 were analyzed by using an anti-MMP-7 antibody (RM7C, red) with an anti-CD45 antibody, anti-Ly6G antibody, or anti-F4/80 antibody (green). Nuclei were labeled in DAPI blue fluorescence. The zoomed-in merged image was shown on the right. The (B) MMP-7⁺ CD45⁺ cells, (D) MMP-7⁺ Ly6G⁺ cells, and (F) MMP-7⁺ F4/80⁺ cells were indicated by white arrows. Images were taken at 40X magnification through LSM 510 confocal microscope