Fig. 3

The Multi-Plex analysis demonstrates that 300 mg/kg PD increases the cytokine level of IL-6 in colonic tissues in the DSS-induced colitis mouse model. Colonic tissues from healthy (Cont.) and DSS-exposed mice treated with or without PD (150 mg/kg and 300 mg/kg) were lysed and diluted to a final concentration of 1 mg/ml. The level of mIL-1β, mIL-6, mIL-10, mIL-22, mTNF-α, and mIP-10 in 1 mg/ml colonic lysates were measured through the Multi-Plex immunoassay using Bio-Plex® 200 system. Data are represented as mean ± SEM, n = 5 mice. *p < 0.05. PD, Pulsatilla decoction