Fig. 1

Effects of Pulsatilla decoction (PD) in DSS-induced colitis mouse model. 8-week male C57BL/6 mice were untreated (Cont.) or given with 2.5% DSS in drinking water for 8 days. Daily oral administration of PD (150 mg/kg and 300 mg/kg) were started on day 4. (A and B) Body weights were monitored daily and presented as a percentage of initial weight. (C) Disease activity index (DAI) scores were calculated as described in Table 1. (D) Colon lengths were measured on day 8 after sacrifice. The representative images of the colon of each experimental group were shown on the right. (E) The images of colon sections from healthy (Cont.) and DSS-exposed mice treated with or without PD (150 mg/kg and 300 mg/kg) were stained with hematoxylin and eosin (H&E). Images were taken at 20X magnification (scale bar: 50 μm, shown in the upper right) and 40X magnification (scale bar: 20 μm). (F) Histology scores of colon sections were calculated as described in Table 2. Data are represented as mean ± SEM, n = 5 mice. *p < 0.05, **p < 0.01, ***p < 0.001. PD, Pulsatilla decoction