Human liver microsomes study on the inhibitory effect of plantainoside D on the activity of cytochrome P450 activity

Table 1 Isoforms tested, marker reactions, incubation conditions, and K_m used in the inhibition study

CYPs	Marker reactions	Substrate concentration (μM)	Protein concentration (mg/mL)	Incubation time (min)	Estimated K_m (μM)	Inhibitor concentration (μM)	Inhibitors	Reference
1A2	phenacetin O-deethylation	40	0.2	30	48	10	furafylline	[16, 17]
2A6	coumarin 7-hydroxylation	1.0	0.1	10	1.5	10	tranylcypromine	[16, 17]
2B6	Bupropion to hydroxybuptopion	40	0.25	30	50	50	monoterpenoid	[18, 19]
2C8	paclitaxel 6α-hydroxylation	10	0.5	30	16	5	montelukast	[16, 17]
2C9	diclofenac 4’-hydroxylation	10	0.3	10	13	10	sulphaphenazole	[16, 17]
2C19	S-Mephenytoin 4-hydroxylation	100	0.2	40	105	50	tranylcypromine	[16, 17]
2D6	dextromethorphan O-demethylation	25	0.25	20	4.8	10	quinidine	[20]
2E1	chlorzoxazone 6-hydroxylation	120	0.4	30	126	50	clomethiazole	[16, 17]
3A4	testosterone 6β-hydroxylation	50	0.5	10	53	1	ketoconazole	[16, 17]

ISSN: 2662-7671