Cytotoxicity, acute and sub-chronic toxicities of the fruit extract of Tetrapleura tetraptera (Schumm. & Thonn.) Taub. (Fabaceae)

Table 1 Cytotoxicity of extracts, compounds and doxorubicin towards human melanoma and other animal cancer cell lines as determined by RRA

Features and cell lines		IC₅₀ values (μM)
Features and cell lines		TTF	2	5	6	7	Doxorubicin
BRAF-V600E homozygous mutant melanoma	MaMel-80a	30.18 ± 2.89	44.28 ± 3.29	10.02 ± 0.21	28.67 ± 3.12	29.08 ± 1.48	8.66 ± 0.56
BRAF-V600E homozygous mutant melanoma	SKMel-28	28.23 ± 1.72	67.28 ± 5.12	31.73 ± 2.20	33.95 ± 2.53	33.11 ± 2.19	2.14 ± 0.12
BRAF-V600E heterozygous mutant melanoma	A2058	19.48 ± 0.82	78.76 ± 5.65	22.77 ± 1.72	> 150	79.42 ± 4.41	0.29 ± 0.04
BRAF-V600E heterozygous mutant melanoma	Mel-2a	18.96 ± 0.69	102.56 ± 8.94	25.45 ± 3.01	109.24 ± 8.44	> 150	6.63 ± 0.41
BRAF wildtype melanoma	MV3	28.72 ± 3.11	78.33 ± 6.29	23.52 ± 1.27	> 150	> 150	7.09 ± 0.59
BRAF wildtype melanoma	SKMel-505	36.18 ± 2.62	> 150	22.18 ± 2.01	88.74 ± 6.42	> 150	9.39 ± 1.01
Rat colon adenocarcinoma	CC531	18.95 ± 1.27	54.39 ± 3.63	10.21 ± 0.14	66.38 ± 4.77	54.11 ± 3.10	0.44 ± 0.23
Murine melanoma	B16-F1	18.32 ± 0.96	32.20 ± 2.17	24.08 ± 0.99	44.90 ± 5.03	69.04 ± 4.95	0.22 ± 0.01
Murine melanoma	B16-F10	20.04 ± 1.45	41.85 ± 3.69	24.64 ± 2.56	50.12 ± 3.76	61.45 ± 5.21	0.24 ± 0.03

TTF: dichloromethane-methanol extract of TTF; 1: (3R, 4S)-3,4-dimethyloxetan-2-one; 2: luteolin; 3: stigmasterol; 4: 3-O-[6’-O-stearoyl-β-_D-glucopyranosyl]stigmasterol; 5: olean-12-en-3-β-O-_D-glucopyranoside; 6: 3-O-β-_D-glucopyranosyl-(1 → 6)-β-_D-glucopyranosylurs-12-en-28-oic acid; 7: 3-O-β-_D-glucopyranosyl-(1 → 3)-β-_D-glucopyranosyl-27-hydroxyolean-12-en-28-oic acid; 8: methyl-O-β-_D-glucopyranoside; 9: β-_D-fructofuranosyl-(1 → 4)-β-_D-glucopyranoside. Compounds 1, 3, 4, 8 and 9 were not active, with no observable IC₅₀ at up to 150 μM. In bold: significant cytotoxic effect (Boik, 2001; Brahemi et al., 2010; Kuete and Efferth, 2015); Boik, 2001; Brahemi et al., 2010)

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