Fig. 3

Ajwain nsLTP1 mechanism of action in inducing apoptosis in MCF-7 and AsPC-1. Both cell lines were treated with the same concentrations and compared to control cells. A In MCF-7, nsLTP1 promoted apoptosis by increasing the gene expression of proapoptotic proteins (Bax and Caspase-3) and decreasing the anti-apoptotic protein (Bcl-2). Also, it decreased the expression of other cancer-associated genes that promote cell survival, including, Survivin, EGFR, and VEGF. B In AsPC-1, nsLTP1 promoted apoptosis by upregulation of the gene expression of Bax and Caspase-3, and downregulation of Bcl-2, Survivin, EGFR, and VEGF. All data were obtained from three independent experiments and significance are indicated by *P < 0.05, **P < 0.01, ***P < 0.001