Fig. 2

The apoptotic effect of Ajwain nsLTP1 using Annexin V-FITC/PI staining by flow cytometry. Live cells (Annexin V−, PI−), early apoptotic cells (Annexin V+, PI−), late apoptotic cells (Annexin V+, PI+), and necrotic cells (Annexin V−, PI+), (A) MCF-7 (B) AsPC-1 cells. The percentage of apoptosis among different concentration treatments is represented in MCF-7 cells (C) and AsPC-1 cells (D) compared to control cells. The sum of early apoptotic and late apoptotic cells was considered as the percentage of apoptosis. The data were presented as the mean ± SD of three independent experiments and significance are indicated by *P < 0.05, **P < 0.01, ***P < 0.001