Fig. 4

TCA restored calcium handling and sarcomere contractility. A-B Representative immunofluorescence images of p-PLN and p-RyR2. Green: p-PLN and p-RyR2; Blue: DAPI; C Diagrammatic sketch of the calcium sparks. D Average traces of Ca²⁺ transients (above) and cell shortening (down) from Fluo-4/AM and F-127 loaded cardiomyocytes isolated from the heart of adult mice. E Quantification of Ca²⁺ spark frequency, Ca²⁺ transients and cell shortening. *P < 0.05 versus control, #P < 0.05 versus PE. PLN: phospholamban; RyR2: ryanodine receptor type 2