Fig. 2

The proangiogenic effect of CDDP on subintestinal vessels (SIVs). A Twenty-four hpf embryos were treated with various concentrations of CDDP (0.3, 0.6, 0.9 and 1.2 mg/mL) for 48 h. The survival rate was recorded. B The toxicity screening of CDDP did not significantly affect the early-stage appearance of embryos. Scale bar, 50 μm. C Body length was not affected by CDDP treatment, indicating that embryo development was normal. D The yolk sac area was also unchanged, which meant that the nutritional status was maintained at a similar level. E Heart rates of the treatment groups were not notably different from those of the control group. F Schematic representation of the experimental design for G-, I. Twenty-four hpf transgenic zebrafish Tg(fli1: EGFP) embryos were cotreated with CDDP (0.3, 0.6, 0.9 and 1.2 mg/mL) for 24 h. After washing with culture water, the embryos were treated with the same concentration of CDDP for another 24 h. The box in 72 hpf larvae indicates the position photographed and displayed in G. G Representative pictures of each group; arrows indicate branch points and sprouting vessels of SIVs induced by CDDP in zebrafish. Scale bar, 50 μm. H, I The branch point and sprouting vessel numbers of SIVs were recorded in each zebrafish. Fifteen embryos were included in each group, and the experiment was replicated four times. Data are represented as the mean ± SEM