Fig. 5

HF induces autophagy in K562 cells. A, B The expression levels of LC3-II and p62 proteins in K562 cells after treatment with different concentrations of HF (0, 5, 10 and 20 μM) and different times of 10 μM HF. C, D The expression levels of proteins in K562 cells pre-treated with 1 mM 3-MA or 5 μM CQ for 1 h before exposed to 10 μM HF for 48 h. E The cell viability of K562 cells measured by CCK-8 assay. F The expression levels of LC3-II and p62 in K562 cells pre-treated with 10 μM SP600125 for 1 h before exposed to 10 μM HF for 48 h. *P < 0.05, **P < 0.01, ***P < 0.001