Fig. 6

S. jambos extract regulates the ET-1 stimulated ROS production in endothelial cells. A Human endothelial cells were incubated for 1 h with different concentrations (from 1 to100 μg/ml) of S. jambos extract or PBS as vehicle (0 μg/ml S. jambos) followed by 100 nM ET-1 stimulation for 1 h. ROS was determined using CM-H2DCFDA fluorescence as described in methods. Values represent the mean ± SD of n = 4. *, p < 0.02; **, p < 0.004 ***, p < 0.009. ET-1 treated samples in absence vs. presence of S. jambos extract. ^#p < 0.001 vehicle vs ET-1. B-C comparison between Trolox (200 μM) and S. jambos (50 μM/mL) ROS inhibitory capacity. To determine the antioxidant capacity of S. jambos extract, we used Trolox as a positive control. Trolox equivalent antioxidant capacity (TEAC) for S. jambos extract was 54%. Values represent the mean ± SE (n = 5). #p < 0.001, ###p < 0.00002, Trolox vs S. jambos in the present of ET-1. ##p < 0.001, vehicle vs ET-1