Fig. 2

Effects of resin glycoside and its components on tonus of colon isolated from mice. A, representative record showing effect of pharbitin, phar-genin, pharbitic acid and acetylcholine (ACh) on tonus of colon. The grey vertical line shows the start of sample administration. B, normalized amplitude and area of colon tonus in the presence of pharbitin. C, normalized amplitude and area of colon tonus in the presence of phar-genin. Amplitude and area of colon tonus in the presence of pharbitin or phar-genin were normalized to mean amplitude and area recorded in pre-control (resting tonus), respectively. D, Normalized amplitude and area of colon tonus in the absence or presence of pharbitin and atropine. At first, colon tonus in Tyrode’s solution was recorded for 5 min as a pre-control. After stable, colon ring was pretreated with atropine of 10 μM for 3 min, and then treated with pharbitin of 30 μg/mL for 5 min