Fig. 2
Extending the incubation time enhanced the IC50 for all SRCC cell lines. Influence of incubation time with AA on non-SRCC cells (GPM-1), patient-derived gastric origin SRCC (GPM-2), gastric-origin SRCCs (HSC-39 and KATO-3), and human pancreatic (MIA PaCa-2) and ovarian (SKOV-3) lines. A Inhibition curves of A among various cells after incubations of 1, 16 and 72 h, respectively. B IC50 of AA after 1, 16 and 72 h on SRCC (left) and non-SRCC (right) (n = 3). Data were analyzed by two-way ANOVA. P values < 0.05 were considered statistically significant. Significance is reported for the difference between the labeled groups (i.e., 16- or 72-h) and 1-h treated group (* p < 0.05, ** p < 0.01, ** p < 0.001, **** p < 0.0001). C Cell viability after incubation with AA. After treatment, cells were stained with calcein and scanned on a Celigo imaging cytometer