Fig. 4

Inflammatory marker levels decreased following A. argyi extract treatment in mice with DSS-induced colitis. a The protein expression levels of inflammation-related markers, including phospho-IκB and phospho-NF-κB, and Cox2, were determined using western blotting in mouse colon tissue. Band densities of phospho-IκB and Cox2 were normalized to that of β-actin and the density of phospho-NF-κB was normalized to that of NF-κB. b mRNA expression of inflammation-related genes, including IL-1β, TNF-α, ICAM-1, MCP1, and iNOS. The mRNA level was normalized using GAPDH. c The protein expression levels of Nrf2 and its target protein HO-1 were detected using western blotting in mouse colon tissue. Band densities of Nrf2 and HO-1 were normalized to that of β-actin. Data are presented as mean ± SEM from three independent experiments. Different superscripts indicate significant differences at P < 0.05. CON, the mice group was treated with pure water; DSS, the mice group was treated with 3% DSS solution; ASA, the mice group was treated with 3% DSS solution and 100 mg kg-1 day-1 of 5-amino salicylic acid (5-ASA); AA, the mice group was treated with 3% DSS solution and 200 mg kg-1 day-1 of A. argyi extract