Fig. 1
Anti-inflammatory and anti-oxidative effect of A. argyi on LPS-induced RAW 264.7 cells. a, b Amounts of nitric oxide (NO) and prostaglandin E2 (PGE2) were measured using the supernatant of RAW 264.7 cells. Cells were treated with the A. argyi extract (0–80 μg/mL) for 24 h. c Protein expression levels of Cox2 and iNOS were determined using western blotting in LPS-induced RAW 264.7 cells. The cells were treated with 0, 10, 20, and 40 μg/mL of the A. argyi extract for 24 h. d The protein expression level of NF-κB accumulation in the nucleus. The cells were treated with the A. argyi extract (0, 10, 20, and 40 μg/mL) for 24 h. e–f The protein expression levels of Nrf2 and its target gene HO-1 were determined using western blotting in LPS-induced RAW 264.7 cells. The cells were treated with 0, 10, 20, and 40 μg/mL of the A. argyi extract for 24 h. Data are presented as mean ± standard error of the mean (SEM) from three independent experiments. Different superscripts indicate significant differences at P < 0.05