Fig. 4

CF attenuated Wnt/β-catenin/RAS signaling activation in the kidney of SAMP8 mice. Scale bars: 50 μm. The localization of AGTR1, Wnt4, β-catenin, and CTGF in the kidney was detected by immunohistochemistry (a), and β-catenin (b) and CTGF (c) were semi-quantitatively analyzed. Protein strip (d) and quantification of renin (e), Wnt4 (f), and AGTR1 (g) in the kidney were detected by western blotting. Con: SAMR1 mice; M: SAMP8 mice; CF-L: Treated with low-dose CF ethanol extract; CF-M: Treated with medium-dose CF ethanol extract; CF-H: Treated with high-dose CF ethanol extract; Arrows indicate positive expression. Data represent the mean values ± SD. ^*P < 0.05 and ^**P < 0.01 vs. the M group (n = 3)