Fig. 3
From: In vitro evaluation of the inhibition potential of echinacoside on human cytochrome P450 isozymes
Time-dependent inhibition of ECH on CYP3A4. Microsomal reaction mixtures were pre-incubated for 0, 5, 10, 15, 30 min with or without ECH at 0, 2, 5, 10, 20, 50 μM. A The inhibitions of ECH on CYP1A2, CYP2E1, and CYP2C19 were time-independent, while the inhibition of ECH on CYP3A4 was time-dependent. B ECH inhibited CYP3A4 in a time-dependent manner. C The value of Kobs is plotted against the concentration of the inactivator to determine KI and Kinact. ECH: echinacoside