Skip to main content
Fig. 4 | BMC Complementary Medicine and Therapies

Fig. 4

From: Ononin ameliorates inflammation and cartilage degradation in rat chondrocytes with IL-1β-induced osteoarthritis by downregulating the MAPK and NF-κB pathways

Fig. 4

Effects of Ononin on IL-1β-induced ECM degradation in Rat Chondrocytes. (A) Expression levels of Collagen II and MMP-13 were detected by Western Blot. The samples derive from the same experiment and that different gels were processed in parallel. (B, C) The expression level of target proteins was expressed by the ratio of grey value of target band to GAPDH band by Image-J software. (D) Immunofluorescence staining of Collagen II protein (green) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). (E) Immunofluorescence staining of MMP-13 protein (red) and nucleus (blue) was labelled with DAPI and photographed by fluorescence microscope (20×) (scale bar: 50 μm). (F, G) The summary data of fluorescence intensity of Collagen II and MMP-13 in situ. All experiments were performed in 3 times and data were expressed by mean ± SD. *P < 0.05 compared with control group; #P < 0.05 compared with IL-1β group; **P < 0.01 compared with control group; ##P < 0.01 compared with IL-1β group

Back to article page

BMC Complementary Medicine and Therapies

ISSN: 2662-7671

Contact us