Fig. 1
Screening of extracts for immunomodulatory activity. Crude extracts of S. cordifolia (SC) were prepared by sequential extraction using n-hexane (SCHEX), chloroform (SCCL), methanol (SCMEX) and water (SCAQ). Each crude extract was screened for immunomodulating activity using murine splenocyte proliferation (Alamar Blue assay), splenocyte antibody production, macrophage phagocytosis of neutral red and macrophage respiratory burst activity indirectly by measuring NO2− production using Griess assay). Immunostimulatory activity in order from highest to lowest is red > yellow > blue