Fig. 5
Effects of Quercus acuta Thunb. fruit (QAF) on activator protein 1 (AP-1) and mitogen-activated protein kinase (MAPK) signaling pathways in UVB-induced HaCaT keratinocytes. (A) The effects of QAF on the phosphorylation of MAPK activated by UVB. Cells were seeded and treated with a range of concentrations of QAF (5, 10, 20, and 50 μg/mL) for 24 h. Cells were irradiated with UVB at a dose of 30 mJ/cm2 and harvested 30 min later. Protein expression was evaluated using western blotting, and (B) the band intensities were quantified. (C) The effects of QAF on the phosphorylation of AP-1 activated by UVB. Cells were treated with a range of concentrations of QAF (5, 10, 20, and 50 μg/mL) for 24 h, irradiated with UVB at a dose of 30 mJ/cm2, and harvested 10 h later. Protein expression was evaluated using western blotting, and (D) the band intensities were quantified. Data are expressed as the mean ± SD. #p < 0.05, ##p < 0.01, ###p < 0.001 versus control group; *p < 0.05, **p < 0.01, ***p < 0.001 versus UVB-treated group