Fig. 2

Construction of TRPC6 overexpressing cell model, and detection of Ca²⁺ influx among groups. The mRNA and protein levels of TRPC6 were determined by qRT-PCR (a) and western blotting (b), respectively. The intracellular Ca2⁺ influx was determined using Fluo-3 AM under confocal microscope (c) and semiquantitative analysis (d). Different letters represent statistically different values (P < 0.05), whereas the same letters indicate no significant difference