Fig. 4

Lung tissue specimens collected from immunosuppressed mice on the second day after death from invasive C. albicans infection were prepared for observations by transmission electron microscopy (TEM) and electron microscopy. a The mycelial cell wall had a plate-like multilayer thick-walled structure with a clear cell membrane and complete contents. b The budding yeast cell wall had a multi-layer thick-walled structure with a clear cell membrane and complete contents. c The outer layer of the yeast cell wall of C. albicans dissolved and fell off, the cell wall became thinner, and the cell nuclei and organelles dissolved and disappeared. However, the cell membrane was clear and intact. d The outer layer of the cell wall of the hyphae of C. albicans dissolved and fell off, the cell wall was notably thinned and severely damaged, and the cell nuclei and organelles dissolved and disappeared. e After 14 days of fluconazole to treat invasive C. albicans infection in immunosuppressive mice, lung tissue specimens were collected and prepared for transmission electron microscopy (TEM). The following were observed under the electron microscope. The yeast cell wall of C. albicans was relatively complete, but the cell membrane was completely shed and the cell nuclei and organelles dissolved and disappeared