Fig. 8

Neuroprotective effects of SZJN prescription against glutamate-induced PC12 cell death. Cells were induced by glutamate solution (22.5 mM) and treated with SZJN prescription (0.05, 0.1 and 0.2 mg/mL) and MH (10 μM) containing the fluorescent dye YOYO-1 to identify dead cells for over 24 h using IncuCyte instrument (n = 3). Cells were imaged every 2 h for 24 h on the Essen Bio Science IncuCyte. a Time course cell viability analysis of PC12 cells using IncuCyte instrument. b-d Time course evaluation of total green fluorescent area, the number of dead cells and percent cell death over 24 h by YOYO-1 uptake on an IncuCyte instrument. e Representative images of YOYO-1 positive cells at 0, 12, 24, 36 hpt