Fig. 7
From: The ethanol extract of Garcinia subelliptica Merr. induces autophagy
eGSM induced autophagy in the absence of AMPK activity. A The expression of AMPKα1/2 genes was inactivated by CRISPR in HEK293 cells (lanes 4 to 6, bottom panel). Along with WT (lanes 1 to 3), AMPKα KO cells were treated with two different amounts of eGSM for 16 h. Total proteins were isolated and analyzed by western blotting for LC3-1 and LC3-II (top panel), the membrane of which was stripped and reblotted for β-actin (middle panel). B WT (lanes 1 to 3) and AMPKα KO cells (lanes 4 to 6) were treated as in (A), and ULK1 phosphorylated at S317 (top panel), a serine phosphorylated by activated AMPK, and ULK1 (2nd panel) and were analyzed by Western blot. AMPKα and β-actin were similarly analyzed with stripped membranes