Fig. 5

eGSM decreased mTOR activity in SNU2292, not in A549. From A549 cells (A) or SNU2292 cells (C) treated with indicated amounts of eGSM for 16 h (lanes 2 to 4), total proteins were extracted and analyzed for ULK1 phosphorylated at S757, a target of mTOR kinase activity. Rapamycin (10 μM, 6 h) was included as a negative regulator of mTOR kinase activity (lane 5). The blotted membrane was stripped and reprobed for ULK1 to ensure equal loading. The bands were analyzed by ImageJ, and the relative levels of the phosphorylated ULK1 over ULK1 in A549 (B) and SNU2292 (D) are shown, where each band was measured three times and shown as in the mean ± SEM. *P was less than 0.05, compared to untreated controls