Fig. 2

The three active ingredients protected APP-NSC from death and damage. The monolayer cells were cultured in proliferation medium for 24 h, exposed to DISS (20; 40 and 60 μM), OB (2.5; 5 and 10 μM) and TEN (20; 40 and 60 μM), respectively for another 24 h. Then the MTT and LDH assays were carried out. (A-C) The viability of APP-NSCs administrated with DISS (A), OB (B) or TEN (C) were measured by the MTT assay. (D-F) The LDH leakage of APP-NSCs exposed to DISS (D), OB (E) or TEN (F) were detected by the LDH kit. ##P < 0.01 vs. GFP-NSCs group; *P < 0.05; **P < 0.01 vs. APP-NSCs group. Data are represented as mean ± sem. This experiment was performed three times. Data were analyzed by one-way ANOVA