Fig. 1
From: The cytotoxic effect of Baeckea frustescens extracts in eliminating hypoxic breast cancer cells
Induction of hypoxic condition using DMOG. MCF-7 cells were either treated or untreated with DMOG at various concentrations for 72 hours before harvesting and immunoblotted with antibody as indicated. HIF-1α was detected using HIF-1α antibody. Actin served as loading control. Bar chart shows quantification of HIF-1α levels compared to β-actin control at various DMOG concentrations