Fig. 5

PL-induced DNA damage is not repaired in CRPC PC3 and DU145 cells. A Representative images of DNA damage via comet assays. PC3 or DU145 cells were treated with PL (10 μM) for 3 h and were then collected at the indicated times (0, 2, 4, 8, 24, or 48 h). Scale bar, 100 μm. B The percentages of tail DNA in PL-treated PC3 or DU145 cells were measured. Values represent the mean ± SD of at least three independent experiments, and ≥ 500 cells were counted in each group. Statistical significance was calculated using unpaired Student’s two-tailed t tests (*p < 0.05, **p < 0.01, ***p < 0.001)