Fig. 3

miR-134 binds to SLUG mRNA 3’UTR to suppress protein translation. Normalized luciferase activity in 4 T1 cells transfected with pGL3-control-SLUG 3’UTR vector and miR-22, miR-134 chemically synthesized mimics (A) or inhbitors (B). (C) The putative miR-134 binding sites; the wild-type, mutant (Mut) and deletion sequence of 3′-UTR of SLUG. Normalized luciferase activity in 4 T1 cells transfected with various reporter vector constructs with miR-67 or miR-134 mimics (D) or inhibitors (E). ***P < 0.001, ###P < 0.001. P-values were determined by one-way analysis of variance. Data are mean ± standard error of the mean and are representative of three independent experiments