Fig. 6
Ca2+-induced pressure changes were associated with TRPCs channel. A, B, C, D the pressure- Ca2+ response curves of CCA rings of WKY rats with intact endothelium, 10 μM SKF and 200 nM SAR could obviously decrease CCA pressure with endothelium compared to the control; 100 μM ANCOA4 has no effect on CCA pressure. E The pressure-Ca2+ response of CCA rings in WKY rats without endothelium, SKF could obviously decrease CCA pressure without endothelium compared to the control. F, G The pressure-inner diameter curves of CCA of SHR rats incubated with SKF, SAR or ANCOA4 with intact endothelium or without endothelium. H Time course of [Ca2+]i changes as seen in Fura2-loaded A7R5 cells, PUE were incubated for 24 h before experiment, 0.1 μM AngII was used in the experiment. I Fluorescent dye Fluo-3/AM staining of A7R5 cells, Scale bar: 1 mm. SKF: SKF96365, pan-TRPCs inhibitor; SAR: SAR7334, specific TRPC6 inhibitor; ANCOA4:Orai1 inhibitor, * p < 0.05 vs Ctrl. (Two-way ANOVA with Bonferroni post-hoc test)