Fig. 2

mRNA and protein levels of IL-6 and TNFα after essential oil treatments of BV-2 cells. BV-2 cells were treated with 200-fold diluted essential oil chemotypes and standards, and DMSO treatment was used as a control of the treated cells. Real-time PCR was performed with SYBR green protocol using IL-6 and TNFα specific primers. β-actin was used as housekeeping gene for the normalization and relative expression of controls was considered as 1. Proinflammatory cytokine secretions were determined using IL-6 or TNFα specific ELISA kits according to the manufacturer’s protocols. a Relative mRNA expressions of IL-6 and TNFα of the essential oil treated cells. b IL-6 and TNFα ELISA measurements from the supernatant of the essential oil treated BV-2 cells. The bars represent mean values and error bars represent standard deviation (SD) for three independent determinations (n = 3). Real-time PCR and ELISA measurements were carried out in triplicate in each independent experiments. Asterisks indicate p < 0.05 compared to the control