Fig. 3

Immunohistochemistry of IL-1β protein (A-D). Multifocal inflammatory cells express inflammatory markers (arrows) in the control group (A). Inset a: higher magnification with intensive cytoplasmic expression. Immunostaining of mononuclear inflammatory cells (arrow) at the center of granuloma (B). No immunolabeling of inflammatory cells in SME1%(C) and SME 5% (D). The panel of anti iNOS antibody expression (E-H). Multifocal immunopositivity of inflammatory cells throughout the lesion (arrows) in the control group (E). Inset e: strong positive cells in higher magnification. Multifocal immunoexpression of marker (arrows) in peritoneal adhesion inflammatory cells (F). Multifocal with a lower population of cells’ immunolabeling (arrows) in contrast to the F group is presented (G). Inset g: aggregated intensive cells are immunostained (arrow). Highly dispersed mononuclear cells in a narrow rim of adhesive connective tissue (H). Immunostaining of COX-2 panel (I-L). The high density of inflammatory cells which have a cytoplasmic reaction (arrow) in control (I). Inset i: compressed and intense immunolabelled cells (arrow). Multifocal positive reaction in a condensed population of inflammatory cells (arrows) (J). Inset j: strong cytoplasmic staining (arrow). Moderate inflammatory cells but strongly expressed against antibody (arrows) (K). Inset k: cytoplasmic positive cells are demonstrated at the center of inflammation. Scattered point cells might be confined at the thin marginal surface (L). Inset l: undetectable inflammatory cells at higher magnification. The panel of anti-vimentin protein (M-P). Strong Multifocal reaction to antibody specified by arrows in the control group (M). Inset m: higher magnification of vimentin reaction in mesenchymal cells. Large numbers of connective tissue cells are immunolabeled against antibody (arrows) (N). Inset n: the cells are well demonstrated in this field (arrow). Very few populations of cells have a positive reaction to the antibody (arrow) (O). Inset o: More pronounced expressed cells are determined (arrow). No immunostaining cells are detected in a high dose of SME (P). A-P × 40. Inset: a, e, k, m (× 400) & g, i, j, l, n, o (× 100)