Fig. 2

Screening of selected compounds for migrastatic effects on RH-30 cells. a Evaluation of cell migration capacity and relative migration speed (ratio) of RH-30 cells during VSM (10 and 25 μg/ml), LW (50 μg/ml), Geni (100 μM) and Jaspl. (1 nM) treatment for 2 days compared to the control (0.1% DMSO). Mean ± SD, n = 6–8, *** P < 0.001, ** P < 0.01, * P < 0.05, significantly different compared to vehicle control, unpaired t-test. b Determination of the relative invasion capacity of RH-30 cells after 48 h VSM (10 and 25 μg/ml), Geni (50 μM) and Jaspl. (10 nM) treatment compared to the vehicle control (0.1% DMSO). Mean ± SD, n = 3, *** P < 0.001, ** P < 0.01, * P < 0.05, significantly different compared to vehicle control, unpaired t-test. c Determination of the relative colony formation capacity of RH-30 cells after 48 h VSM (25 μg/ml), Geni (50 μM) and Jaspl. (10 nM) treatment compared to the vehicle control (0.1% DMSO). Mean ± SD, n = 3, *** P < 0.001, ** P < 0.01, * P < 0.05, significantly different compared to vehicle control, unpaired t-test. a-c Note the individual adjustments in the treatment concentrations of Jaspl. and Geni used within Figs. 2a, b and c are to optimize the respective assay conditions to capture respective minimum effective initial concentration in each graph and to ensure assay function, respectively