Table 1 Sequences of primers used for real-time qPCR
From: Alisol A 24-acetate stimulates lipolysis in 3 T3-L1 adipocytes
Gene name | Forword primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
β-actin | CGCTCGTTGCCAATAGTG | GCTGTGCTATGTTGCTCTAG |
MGL | CGGACTTCCAAGTTTTTGTCAGA | GCAGCCACTAGGATGGAGATG |
HSL | AGACCACATCGCCCACA | CCTTTATTGTCAGCTTCTTCAAGG |
ATGL | GACCTGATGACCACCCTTTC | TGTTTGGCTTTATCTCGGCTC |
PPARγ | CAAGAATACCAAAGTGCGATCAA | GAGCTGGGTCTTTTCAGAATAATAAG |
Perilipin A | GCTCTTCAATACCCTCCAGAAAAG | TTCGAAGGCGGGTAGAGATG |
PGC-1α | CCCTGCCATTGTTAAGACC | TGCTGCTGTTCCTGTTTTC |
UCP-1 | CCTGCCTCTCTCGGAAACAA | GTAGCGGGGTTTGATCCCAT |
PPARα | AACATCGAGTGTCGAATATGTGG | CCGAATAGTTCGCCGAAAGAA |