Fig. 5

MabE inhibits IMQ-induced activation of IL-17A-producing γδT cells. Balb/c mice were treated with IMQ cream for 6 consecutive days and splenocytes were collected. a Cells were subjected to flow cytometry analysis and the representative dot plots of lymphocytes electronically gated on CD3ε⁺ cells are shown. The numbers are the percentages of cells electronically gated on γδT cells (γδTCR⁺ CD4⁻) and CD4⁺T cells (γδTCR ⁻ CD4⁺). The summary (n = 4 mice/ group) of percentages (b) and cell numbers (c) of γδT cells is shown. d Cells were (2 × 10⁶ cells/well) stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 24 h. The cell-free culture supernatants were collected and the production of IL-17A was measured by ELISA. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01 compared with IMQ-treated mice. The presented data are representative of three independent experiments