Fig. 2

Effects of MabE on TLR ligand-induced inflammatory cytokine production in RAW264.7 cells. a RAW264.7 cells were treated with MabE (25 μg/mL) for 1 h and then stimulated with IMQ (20 μg/mL), poly I:C (20 μg/mL) or LPS (100 ng/mL) for 3 h. The mRNA expression levels of interleukin (IL)-6 and IL-1β were quantified by real-time PCR. b The production of IL-6 and IL-1β was measured in the culture medium of cells stimulated with IMQ (20 μg/mL), poly I:C (20 μg/mL) or LPS (100 ng/mL) for 24 h in the presence of 25 μg/mL of MabE. After culture supernatants were collected, each cytokine was quantified using the ELISA kit according to the manufacturer’s instructions. N.D: not detectable