Fig. 4

PB2 inhibits Akt/mTOR pathway in gastric cancer cells. SGC-7901 cells were incubated with PB2 (0, 20, 50 and 100 μM) for 48 h. a Western blot was carried out to determine Akt, p-Akt, mTOR and p-mTOR proteins. b PB2 did not change the expressions of Akt, c but reduced p-Akt protein expression in a concentration-dependent manner. d mTOR remain unchanged after PB2 treatment, e but p-mTOR protein expression is reduced by PB2 in a concentration-dependent manner. ***P < 0.001 vs. ox-LDL group. f PB2-induced reduction in cell viability is further enhanced by Akt inhibitor LY294002. SGC-7901 cells were divided into following groups: Control, LY294002, PB2, LY294002 + PB2, and were cultured for 48 h. Cell viability was detected by CCK-8 assay. ***P < 0.001 vs. control group; ###P < 0.001 vs. PB2 group