Fig. 2

PNS treatment promote EPCs angiogenesis and detection the angiogenesis ability. a CCK8 assay for EPCs cultured in different concentrations of PNS at 24 h, 48 h, 72 h and 96 h. b-c qRT-PCR and WB analysis for the expression levels of VEGF-A, bFGF and VE-cadherin treated with EBM or PNS+EBM medium, respectively. d-e After PNS was used to treat EPCs, tube formation and migration was assessed. The tube length and the number of migrating cells were evaluated by counting 5 random fields at × 200 magnification. **compare to CTRL group, p < 0.01