Fig. 7
From: Inhibition of alpha-synuclein seeded fibril formation and toxicity by herbal medicinal extracts
CMC1 and CMC7 binding activity to α-syn monomers and oligomers. a and b Upper panels: Gel filtration profiles of 100 μM α-syn sample incubated with a CMC1 and b CMC7 at 4:1 M ratio (CMC1: α-syn) for 5 days as described in Materials and Methods using Superdex 200 SE column. Peak 1 (P1) and Peak 2 (P2) represent the oligomers species while Peak 3 (P3) represents the monomeric species. The elution was monitored at absorbance wavelengths of A215,A254 and A280. The inset shows immunoblot analysis of different α-syn species (monomers, dimers, and oligomers) separated from the pooled fractions of P1, P2 and P3. Lower panels: Electron microscopy images showing α-syn species resulted from the indicated peaks. c UV absorbance of α-syn P1, P2 and P3 samples collected from the gel filtration chromatography resulted from the incubation of α-syn with CMC1 (left panel) or CMC7 (right panel). d UV absorbance of CMC1 alone (left panel) and CMC7 (right panel). Samples were placed in a10 mm quartz cuvette and the UV absorbance spectra were recorded from 200 nm to 600 nm