Fig. 5

Effect of CMC1 and CMC7 on performed α-syn fibrils. a and b Th-T binding assay used to measure the fibril content resulted from the incubation of aggregated α-syn for the indicated times in the absence or presence of a CMC1 and b CMC7 using different molar ratios (CMC: α-syn at 6:1, 4:1, and 2:1). The assays were performed in triplicates (Means ± standard deviations are from the average of the triplicates). c Electron microscopy images of negatively stained samples of aged α-syn incubated alone or in the presence of CMC1 and CMC7 (CMC: α-syn at 6:1) for 0 h (upper panels) or 48 h (lower panels) with continuous shaking at 37 °C. Scale bar, 500 nm. d Cell viability of BE(2)-M17 cells was tested using MTT assay. Cells were treated for 48 h with pre-formed α-syn fibrils or with the pre-formed fibrils incubated with CMC7 prior to the addition of MTT. Results are expressed as percentages of the average of the control (i.e., untreated cells). Means ± standard deviations are from the average of 3 wells